Last year, BuzzFeed hit more than 220 employees, or 15 percent of the workforce. On Tuesday, employees of the company's news department responded with the announcement that they were planning to form a union.
"It's not so funny and memes," the organizational committee said in a statement. "Our employees have been organizing for months, and we have legitimate complaints about unfair pay quotas, diversified fluctuations and redundancies, weak benefits, skyrocketing costs, diversity and much more."
More than 40 of those who lost their jobs in the last layoff worked in the company's agencies in New York, Washington, San Francisco and Los Angeles. The cuts included all seven members of the BuzzFeed News national desk and its seven-person security group.
The Murmurs of a Union began among the company's employees in 2015, when a group of employees met with NewsGuild in New York to discuss the issue of pay differential, especially in terms of women and color workers. The group continued its efforts this fall, when more than 50 employees attended a meeting at the NewsGuild office.
The work losses have further motivated the organizers, according to Dominic Holden, a political reporter at BuzzFeed News and a member of the Organizing Committee. Davey Alba, a senior technology reporter, said, at his discretion, that "about 90 percent" of qualified BuzzFeed employees supported the idea.
A spokesman for BuzzFeed did not respond immediately to the organizational work. Ben Smith, editor of BuzzFeed News, said in a statement: "We look forward to meeting with the organizers to discuss a way to voluntarily recognize their union."
BuzzFeed workers have already had success in challenging management. Days after the resignation, about 600 current and former employees signed an open letter written on Medium that pushed Mr. Peretti and other executives to compensate the closed employees for unused holiday and days off as part of their exit packages.
It worked. Hours after the letter was published, Peretti informed the staff that he would provide the request.
So far, the Union effort only involves employees at BuzzFeed News, the division of the company led by editor Ben Smith.  If the company recognizes the association, a certification with NewsGuild follows with a negotiation of who would be covered under a contract.
If BuzzFeed does not recognize the association, the organizers said they would make a choice for election with a national labor union official present to facilitate a secret ballot.
"I'm sure of management," Alba said. "I think they will voluntarily recognize it."
Workers at BuzzFeed News are the latest digital media employees to pursue a union service. So far this year, Vice Media, affiliated with Writers Guild of America, East, has expanded the number of employees counted as unions, and employees of Refinery29 organized a union, also under the Writers Guild banner.
According to a report by Harvard Business Review, 30 digital news sites and newspapers have recognized unions since 2015. "In total, the number of unions in internet publishing has increased 20 times since 2010, the report says.
Mr Peretti is on the record and opposes the idea In 2015, he said, "I don't think an association is right for BuzzFeed."
In an interview with The New York Times in November, Mr. Peretti answered a solution to the fleeting economic landscape that now affects digital media companies: a number of mergers among the best internet publishers, including Vice, Vox Media, Group Nine Media and Refinery29, Peretti argued that if BuzzFeed and these companies were to unite, they would be better able to negotiate favorable terms with powerful technical platforms such as Facebook.
"He clearly understands the value of collective bargaining," Mr. Holden said. "And we agree with him that collective action is crucial to ensuring a right nice deal. "